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). While in the free ChVLig-AMP, the OB domain is reflected clear of the NTase area to fully expose the DNA-binding area above the AMP-binding pocket. The peptide segment that is certainly destined to be the latch is disordered inside the no cost ligase and sensitive to proteolysis, but Get Started this section is protected from proteolysis when ChVLig binds to nicked DNA.
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, involving the NTase and HhH domains. Based upon available structural information, it is obvious that DNA ligases have advanced not less than three different implies of encircling DNA.
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